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TOYOBO ReverTra Ace™ qPCR RT Kit

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MT-MC-FSQ-101

High Efficient Reverse Transcription Kit for Real-time PCR  
 
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  • The optimized RT buffer and Primer Mix enable the highly efficient reverse transcription.
  • The reaction can be completed in 15 min. The protocol does not contain an additional RNase H treatment step to remove residual RNA after reverse transcription (Patent Pending).
  • Suitable for the detection of low-expressing mRNAs. Since the RT buffer is optimized for real-time PCR, the addition of 20% (v/v) of the synthesized cDNA solution to the PCR solution does not inhibit the PCR reaction.


    ReverTra Ace™ qPCR RT Kit is an efficient and convenient kit to synthesize high quality cDNAs for real-time PCR. This kit contains the highly efficient reverse transcriptase "ReverTra Ace™" and a RT buffer optimized for the highly efficient synthesis of short-chain cDNAs suitable for real-time PCR. The protocol is simple, and the reaction can be completed in 15 min.
    ReverTra Ace™ is a mutant-type M-MLV reverse transcriptase shows excellent efficiency.
    ReverTra Ace™ qPCR RT Master Mix (Code No. FSQ-201) is a premix version of ReverTra Ace™ qPCR RT Kit. The master mix reagent (5x) contains the highly efficient reverse transcriptase "ReverTra Ace™" , primers and buffer optimized for highly efficient synthesis of short-chain cDNAs suitable for real-time PCR.


     

  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye


  •  QPS-101T
    • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
    • 50x ROX reference dye    50 µl × 1
  •  
  • QPS-101
    • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
    • 50x ROX reference dye    250 µl × 1
  •  
  • QPS-101X5
    • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
    • 50x ROX reference dye    250 µl × 5




  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye




  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye




  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye