High-quality cDNA can be obtained from cell lysates.
Reduction of dispersion on high-throughput assay.
Various real-time PCR regents can be applied.
SuperPrep™ Cell Lysis & RT Kit for qPCR (Code No. SCQ-101) consists of "Lysis Reagents" and "RT Reagents" for synthesis of cDNA templates for real-time PCR assays. "Lysis Reagents" prepares cell lysates containing RNAs that can be used as templates for reverse transcription. "RT Reagents" contains reagents for reverse transcription, optimized for efficient cDNA synthesis from crude lysates. The synthesized cDNA can be applied to real-time PCR directly. This assay system is suitable for high-throughput assays.
SuperPrep™ Cell Lysis Kit for qPCR (Code No. SCQ-201) is an option of "Lysis Reagents". The cell lysate prepared by "Lysis Reagents" can be applied to one-step real-time PCR.
Hot Start
Probe
2x Reaction mix
20µl total reaction volume
Store at -20oC
50x ROX reference dye
QPS-101T
THUNDERBIRD® Probe qPCR Mix 1 ml × 1
50x ROX reference dye 50 µl × 1
QPS-101
THUNDERBIRD® Probe qPCR Mix 1.67 ml × 3
50x ROX reference dye 250 µl × 1
QPS-101X5
THUNDERBIRD® Probe qPCR Mix (1.67 ml × 3) x 5
50x ROX reference dye 250 µl × 5
Hot Start
Probe
2x Reaction mix
20µl total reaction volume
Store at -20oC
50x ROX reference dye
Hot Start
Probe
2x Reaction mix
20µl total reaction volume
Store at -20oC
50x ROX reference dye
Hot Start
Probe
2x Reaction mix
20µl total reaction volume
Store at -20oC
50x ROX reference dye
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