- High specificity The specificity for the detection of low-copy targets is improved.
Homogeneous amplification The dispersion of PCR efficiency between targets is reduced by a new PCR enhancer.
Broad dynamic range High specificity and effective amplification enable the detection of a broad dynamic range.
Compatibility for various real-time cyclers. The reagent is applicable to most real-time cyclers (i.e. Block type and glass capillary type). Because the passive reference dye is included with this kit, the kit can be applied to real-time cyclers that require a passive reference dye.
Fast PCR This reagent enables amplification using fast cycle condition.
Utilization of dUTP This master mix contains dUTP instead of dTTP. Therefore, the rate of false-positive detection can be reduced by adding uracil-N-glycosylase (UNG). *UNG is not supplied with this kit. Uracil-DNA Glycosylase (UNG), Heat-labile (Code No.UNG-101) can be used.
Utilization of a visible tracking dye This master mix contains visible tracking dye. This dye helps to eliminate pipetting errors, and does not spectrally overlap with fluorescent dyes used for qPCR and will not interfere with real-time detection.
THUNDERBIRD™ Next SYBR® qPCR Mix is a highly efficient 2× Master Mix for real-time PCR using SYBR® Green I. The master mix contains all required components, except primers. The master mix facilitates reaction setup, and improves the reproducibility of experiments. This product is an improved version of THUNDERBIRD™ SYBR® qPCR Mix (Code No. QPS-201). In particular, the reaction specificity and PCR efficiency is enhanced.
Hot Start
Probe
2x Reaction mix
20µl total reaction volume
Store at -20oC
50x ROX reference dye
QPS-101T
THUNDERBIRD® Probe qPCR Mix 1 ml × 1
50x ROX reference dye 50 µl × 1
QPS-101
THUNDERBIRD® Probe qPCR Mix 1.67 ml × 3
50x ROX reference dye 250 µl × 1
QPS-101X5
THUNDERBIRD® Probe qPCR Mix (1.67 ml × 3) x 5
50x ROX reference dye 250 µl × 5
Hot Start
Probe
2x Reaction mix
20µl total reaction volume
Store at -20oC
50x ROX reference dye
Hot Start
Probe
2x Reaction mix
20µl total reaction volume
Store at -20oC
50x ROX reference dye
Hot Start
Probe
2x Reaction mix
20µl total reaction volume
Store at -20oC
50x ROX reference dye
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