รายละเอียดสินค้า

Description

THUNDERBIRD® Probe One-step qRT-PCR Kit is a one-step real-time reverse-transcription polymerase chain reaction (RT-PCR) kit that uses the highly efficient reverse transcriptase “ReverTra Ace®” and Tth DNA polymerase as a PCR enzyme. This product can be used mainly in TaqMan® probe assays. The one-step system is suitable for high-throughput analysis because of its simple reaction setup. In addition, this system can reduce the risk of cross-contamination. The combination of the two enzymes and optimized buffer system enable the effective detection and quantification of a small amount of RNA. This kit can also detect various kinds of RNA with different sequences because it is tolerant of target sequence diversity.

Features

• Rapid and highly sensitive
• Tolerant of target sequence diversity
• Tolerant of PCR inhibitors
• Utilization of dUTP
• Multiplex detection
• Hot start system using antibodies
• Compatibility with various real-time cyclers

 Components

This kit includes the following components for 250 reactions, with 20 µL per reaction. All reagents should be stored at −20°C.

2x Reaction Buff*                2x 1.25 ml 

DNA Polymerase                       125 µl 

RT Enzyme Mix                         125 µl 

50x ROX Reference dye           100 µl

RNase free water                2x 1.25 ml *12× Reaction Buffer contains essential components for the reaction (buffer, salts, dATP, dCTP, dGTP, and dUTP).

Uracil-N-glycosylase is not supplied with this kit.