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Description

THUNDERBIRD®SYBR® qPCR Mix is a highly efficient 2x Master Mix for real-time PCR using SYBR® Green I. The master mix contains all required components, except for ROX reference dye, probe and primers. 50x ROX reference dye is individually supplied with this kit. The master mix facilitates fast reaction setup, and improves the reproducibility of experimental data.

This product is an improved version of  Realtime PCR Master Mix (Code No. QPK-201) . In particular, reaction specificity and PCR efficiency is enhanced.

Features

• The master mix contains anti-Taq DNA polymerase antibodies for hot start technology. The specificity for the detection of low-copy targets is enhanced.
• The dispersion of PCR efficiency between targets is reduced by a new PCR enhancer (Patent pending) in the master mix.    
• High specificity and robust enzyme enable the detection of a broad dynamic range.
• The reagent is applicable to most real-time cyclers (i.e. Block type and glass capillary type) including ABI, Bio-Rad, Roche, and TaKaRa. The 50x ROX reference dye in the kit can be applied to real-time cyclers that require a passive reference dye.

Specifications

• Hot Start
• SYBR I
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20C
• 50x ROX reference dye

Components

• QPS-201T
  • THUNDERBIRD® SYBR® qPCR Mix    1 ml × 1
  • 50x ROX reference dye    50 µl × 1
• QPS-201
  • THUNDERBIRD® SYBR® qPCR Mix    1.67 ml × 3
  • 50x ROX reference dye    250 µl × 1
•  QPS-201X5
  • THUNDERBIRD® SYBR® qPCR Mix    (1.67 ml × 3) x 5
  • 50x ROX reference dye    250 µl × 5